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Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Hendrickx, Barbara; Junca, Howard; Vosahlova, Jolana; Lindner, Antje; Rüegg, Irene; Bucheli-Witschel, Margarete; Faber, Folkert (Life Sciences & Renewable Energy); Egli, Thomas; Mau, Margit; Schlömann, Michael; Brennerova, Maria; Brenner, Vladimir; Pieper, Dietmar H; Top, Eva M; Dejonghe, Winnie; Bastiaens, Leen; Springael, Dirk

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed …

2006-02-01AlgemeenArtikel

Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

Faber, Folkert (Life Sciences & Renewable Energy); Egli, Thomas; Harder, Wim

The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine …

1993-09-01AlgemeenArtikel

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Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

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