IL-4 and IL-13 are prototypic Th2 cytokines that generate an “alternatively activated” phenotype in macrophages. We used high-density oligonucleotide microarrays to investigate the transcriptional profile induced in human monocytes by IL-13. After 8-h stimulation with IL-13, 142 genes were regulated …

The exploitation of the metagenome for novel biocatalysts by functional screening is determined by the ability to express the respective genes in a surrogate host. The probability of recovering a certain gene thereby depends on its abundance in the environmental DNA used for library construction, the …

The analysis of regulatory regions in genome sequences is strongly based on the detection of potential transcription factor binding sites. The preferred models for representation of transcription factor binding specificity have been termed position-specific scoring matrices. JASPAR is an open-access …

Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 …

The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic …

Recently, a role for uncoupling protein-3 (UCP3) in carbohydrate metabolism and in type 2 diabetes has been suggested. Mice overexpressing UCP3 in skeletal muscle showed reduced fasting plasma glucose levels, improved glucose tolerance after an oral glucose load, and reduced fasting plasma insulin levels. …

Doel en methode Doel van dit onderzoek was om antwoord te geven op 2 hoofdvragen, namelijk of er sprake is van krachtverlies van perifere skeletspieren bij COPD-patiënten en wat de aanbevelingen zijn voor een trainingsprogramma. Om dit doel te bereiken is er naar literatuur gezocht op internet en in …

Doel van dit onderzoek was om antwoord te geven op 2 hoofdvragen, namelijk of er sprake is van krachtverlies van perifere skeletspieren bij COPD-patiënten en wat de aanbevelingen zijn voor een trainingsprogramma. Om dit doel te bereiken is er naar literatuur gezocht op internet en in diverse bibliotheken. Er …

The data of this study indicate that the acetate recovery factor, used in stable isotope research, needs to be deteremined in every subject, under similar conditions as used for the tracer-derived determination of substrate oxidation.

The results of this study indicate that whole body metabolic and cardiovascular responses to 140 min of either steady state or variable intensity exercise at the same average intensity are similar, despite differences in skeletal muscle carbohydrate metabolism and recruitment