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Antimicrobial susceptibility testing of Gram-positive and -negative bacterial isolates directly from spiked blood culture media with Raman spectroscopy

H.E. Dekter (Onderzoeker); Claudia Constance Orelio (Onderzoeker); Maarten Morsink (Onderzoeker); S. Tektas (Onderzoeker); B. Vis (Onderzoeker); R. te Witt (Onderzoeker); Willem van Leeuwen (Lector)

Patients suffering from bacterial bloodstream infections have an increased risk of developing systematic inflammatory response syndrome (SIRS), which can result in rapid deterioration of the patients’ health. Diagnostic methods for bacterial identification and antimicrobial susceptibility tests are time-consuming. …

2017Natuur & LandbouwArtikel

Extraintestinal pathogenic Escherichia coli sequence type 131 H30-R and H30-Rx subclones in retail chicken meat, Italy

Arash Ghodousi (Onderzoeker); Celestino Bonura (Onderzoeker); Paola di Carlo (Onderzoeker); Willem van Leeuwen (Lector); Caterina Mammina (Onderzoeker)

Extraintestinal pathogenic Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. Among clinical isolates, ST131, primarily its H30-R and H30-Rx subclones, accounts for most antimicrobial-resistant …

2016-04-06Natuur & LandbouwArtikel

Raman spectroscopy-based identification of nosocomial outbreaks of the clonal bacterium Escherichia coli

J.G. Kusters (Onderzoeker); Willem van Leeuwen (Lector); Kees Maquelin (Onderzoeker)

DNA-based techniques are frequently used to confirm the relatedness of putative outbreak isolates. These techniques often lack the discriminatory power when analyzing closely related microbes such as E. coli. Here the value of Raman spectroscopy as a typing tool for E. coli in a clinical setting was …

2016Natuur & LandbouwArtikel

Discrimination of Aspergillus lentulus from Aspergillus fumigatus by Raman spectroscopy and MALDI-TOF MS

P.E.B. Verwer (Onderzoeker); Willem van Leeuwen (Lector); V. Girard (Onderzoeker)

In 2005, a new sibling species of Aspergillus fumigatus was discovered: Aspergillus lentulus. Both species can cause invasive fungal disease in immune-compromised patients. The species are morphologically very similar. Current techniques for identification are PCR-based or morphology-based. These techniques …

2014Natuur & LandbouwArtikel

Clinical effectiveness of dermal substitution in burns by topical negative pressure

Bloemen, Monica C T; van der Wal, Martijn B A; Verhaegen, Pauline D H M; Nieuwenhuis, Marianne K (Functional Recovery And Quality Of Life After Burns); van Baar, Margriet E; van Zuijlen, Paul P M; Middelkoop, Esther

Previous research has shown clinical effectiveness of dermal substitution; however, in burn wounds, only limited effect has been shown. A problem in burn wounds is the reduced take of the autograft, when the substitute and graft are applied in one procedure. Recently, application of topical negative …

2012-11-01AlgemeenArtikel

Comparative study using phenotypic, genotypic and proteomics methods for identification of coagulase-negative staphylococci

Anne J. M. Loonen (Lid Lectoraat); Arjan R. Jansz; Jeandery N. B. Bergland; Marion Valkenburg; Petra F. G. Wolffs; Adriaan J. C. van den Brule (Lector)

Five methods were compared to determine the best technique for accurate identification of coagulase-negative staphylococci (CoNS) (n=142 strains). MALDI-TOF MS showed the best results for rapid and accurate CoNS differentiation (correct identity in 99.3%). An alternative to this approach could be Vitek2 …

2012-03-16Natuur & LandbouwArtikel

An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS

A.J.M. Loonen (Lid Lectoraat); A.R. Jansz; J. Stalpers; P.F.G. Wolffs; A.J.C. van den Brule (Lector)

Matrix-assisted laser desorption/ionisation time of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method for the identification of bacteria from agar media. Direct identification from positive blood cultures should decrease the time to obtaining the result. In this study, three different …

2011-11-12Natuur & LandbouwArtikel

Acceleration of direct identification of S.aureus versus Coagulase Negative Staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods

A.J.M. Loonen (Lid Lectoraat); A.R. Jansz; H. Kreeftenberg; C. A. Bruggeman; P.F.G. Wolffs; A. J. C. van den Brule (Lector)

To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture …

2010-10-24Natuur & LandbouwArtikel

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